Thursday, January 8, 2015

PRO Scientific Homogenizer Generator Maintenance

Generator Probe Maintenance




  • Never run the generator assembly dry. The generator requires liquid sample/medium to lubricate the bearings during processing.

  • Without the liquid, the PTFE bearings can burn out and cause damage to the generator itself.

  • Make sure you are processing the recommended sample volume for the generator/generator assembly you are using. If you are unsure if this is the correct generator for your application, please contact a sales representative before using the generator.

  • Always begin homogenizing at a low rpm, and then gradually increase the speed to your target level.

  • Please follow the instructions in the manual to ensure that you attach the generator/generator assembly properly to the motor unit. (Request a copy of your manual)

  • We recommend taking apart and cleaning generators on a regular basis.

For additional PRO Homogenizer Motor Unit Maintenance Click Here



Dismantling the PRO Quick Connect Generator Probes and Accessories:

5mm Homogenizer Generator Probe Dismantling 5mm generators
The following instructions are for the dismantling of only 5mm generators ONLY.
  1. Unscrew the rotor knife from the bottom of the rotor shaft. Insert the 1/4" hex key (supplied in the tool kit) into the end of the rotor shaft collar and insert the screwdriver (supplied in the tool kit) into the rotor knife and turn the hex wrench counter clockwise.
  2. Remove the rotor knife from the bottom of the generator tube and collar assembly.
  3. Draw the rotor shaft and rotor shaft collar assembly upwards out of the tube and collar assembly. The PTFE (polytetrafluoroethlyene) washer can be removed from the rotor shaft.
  4. Remove the lower bearing from the bottom of the tube and collar assembly. The lower bearing should be replaced when worn before the rotor knife starts to rub against the side of the stator.
  5. The rotor shaft collar assembly can be removed from the rotor shaft by loosening the set screw located at the side of the rotor shaft collar using the hex wrench end of the screw driver (supplied in the tool kit).




7mm and Larger Generator Probe Dismantling 7mm, 10mm, 20mm and 30mm generators
The following instructions are for the dismantling of all other generators except 5mm.
  1. Unscrew the rotor knife from the bottom of the rotor shaft. Insert the ¼” hex key (supplied in the tool kit) into the end of the rotor shaft collar and insert the screwdriver end into the rotor knife and turn the hex wrench counter clockwise.
  2. Remove the rotor knife from the bottom of the generator tube and collar assembly
  3. Remove the rotor shaft collar assembly by loosening the setscrew located in the side of the rotor shaft collar from the rotor shaft using the hex wrench end of the screwdriver tool (For convenience, do not fully remove the setscrew from within the rotor shaft collar assembly).
  4. Remove the PTFE washer from the rotor shaft. Draw the rotor shaft downwards out of the tube and collar assembly. (If rotor shaft does not slide out, press down on the rotor shaft from the top of the generator tube and collar assembly using the hex wrench end of the screwdriver tool).
  5. Remove the lower bearing from the end of the tube and collar assembly using the screwdriver. The screwdriver should be inserted high enough to reach the inner side of the lower bearing. Put the flat side of the screwdriver against the lower bearing, and then pull the handle of the screwdriver against the saw-teeth or open-slotted end of the generator probe. The lower bearing should come out. The lower bearing should be replaced when it shows signs of wear and before the rotor shaft collar starts to come in contact with the inside wall of the motor collar or starts to rub on the top of the tube and collar assembly.




Multi-Gen 7 Generator Probe Dismantling the MULTI-GEN 7 ONLY
  1. Insert the blade end of the screwdriver (supplied in the motor unit tool kit) into the bottom of the generator, locking the knife in place.
  2. Continue with step 3.

Multi-Gen 7XL Generator Probes Dismantling the MULTI-GEN 7XL ONLY
  1. Insert the allen wrench end of the screwdriver (supplied in the motor unit tool kit) across and through the open slot of the XL generator tube
  2. Continue with step 3.
  3. While firmly holding the drive collar at the upper end of the generator (opposite end of the screwdriver) turn the screwdriver counter clockwise. If the drive collar is not held securely it will spin with the screwdriver, thereby not allowing the knife assembly and rotor shaft to be unscrewed from the drive collar.
  4. With the drive collar removed, the rotor shaft knife assembly will slide out the bottom of the generator tube.
  5. The upper PTFE bearing can now be removed from the upper end of the generator tube.
  6. The lower PTFE bearing can now be removed from the lower end of the generator tube.
  7. Multi-Gen parts can now be placed on a tray and autoclaved as necessary.




Assembly of the PRO Quick Connect Generator:

5mm Homogenizer Generator Probe Assembling 5mm generators
The following instructions are for the assembly of only 5mm generators.
  1. Insert the lower bearing into the bottom of the tube and collar assembly. Take the rotor knife and place it into the bottom of the tube and collar assembly and push the lower bearing into its proper location. The proper location is when the end of the rotor knife is flush with the bottom of the tube and collar assembly.
  2. Attach the rotor shaft collar to the end of the rotor shaft. Make sure that the setscrew in the rotor shaft collar lines up with the flat on the end of the rotor shaft. The rotor shaft collar should be located as close to the end of the rotor shaft as possible. Slide the PTFE washer up the rotor shaft until it contacts the rotor shaft collar.
  3. Insert the rotor shaft with the rotor shaft collar and PTFE washer attached into the upper end of the tube and collar assembly.
    • The rotor shaft should rotate freely within the tube and collar assembly. If the rotor shaft does not rotate freely, remove the rotor shaft from the tube and collar assembly and inspect both the upper and lower bearings for any possible damage. Replace any damaged bearings.
  4. Insert the rotor knife into the end of the tube and collar assembly and rotate the knife clockwise while holding the rotor shaft collar.
  5. Once the rotor knife is threaded onto the end of the rotor shaft, insert the ¼” hex wrench into the end of the rotor shaft collar and the screwdriver into the end of the rotor knife and lightly tighten.
  6. With the rotor knife attached, place the generator with the blade end downward onto a flat surface. Loosen the setscrew located on the side of the rotor shaft collar. Push the rotor shaft collar downward until the nylon washer is in contact with both the bottom of the rotor shaft collar and the top of the upper bearing. Tighten the set screw and check that the generator rotates freely.

7mm and Larger Generator Probe Assembling 7mm, 10mm, 20mm and 30mm generators
The following instructions are for the assembly of all other generators except 5mm.
  1. Slide the lower bearing onto the rotor shaft.
  2. Attach the rotor knife to the rotor shaft by screwing it together until tight (hand tighten).
  3. Insert the Rotor Shaft into the end of the tube and collar assembly. Then push the rotor knife up into the end of the tube and collar assembly until it cannot go any further. This will put the lower bearing into its proper place. The rotor shaft should stick out through the upper bearing located in the top of the tube and collar assembly.
  4. While pushing against the rotor knife, place the PTFE washer over the end of the rotor shaft and put the rotor collar assembly onto the rotor shaft.
  5. While holding the rotor knife, align the setscrew on the side of the rotor shaft collar and the flat end on the rotor shaft so they are facing each other. Once lined up with one another, tighten the setscrew against the flat end of the rotor shaft using the hex wrench end of the screwdriver tool (supplied in the tool kit).
  6. Insert the ¼” hex key (supplied in the tool kit) into the end of the rotor shaft collar and insert the screwdriver end into the rotor knife and turn the hex wrench clockwise to confirm its tight (Over tightening of the rotor knife onto the rotor shaft can result in breaking of the rotor shaft and/or distorting of the rotor knife).


Multi-Gen 7 Generator Probe Assembly of the MULTI-GEN GeneratorMulti-Gen 7XL Generator Probes

  1. Insert upper PTFE bearing (bearings are interchangeable) into the upper end of the generator tube-bearing, flange out.
  2. Place the lower PTFE bearing into the lower end of the generator tube- bearing flange out.
  3. Slide shaft into the lower end of the generator tube until knife is flush with tube, making sure threaded end of shaft comes through the upper PTFE bearing.
  4. With finger keeping knife flush with bottom of tube, hand tighten drive collar.
  5. FINAL ASSEMBLY STEP FOR MULTI-GEN 7 ONLY: Place the screwdriver into bottom of the generator and holding the drive collar turn the screwdriver clockwise.
  6. FINAL ASSEMBLY STEP FOR MULTI-GEN 7XL ONLY: Place allen wrench across and through the open slot of the XL generator to ensure tightness of assembly and holding drive collar turn screwdriver clockwise.



Homogenizer Accessory Cleaning
  1. Immediately after you have finished working with the generator, the generator must be cleaned so that the substance residues do not stick to the rotor and stator and allow small bacterial cultures to form in undesirable places.
  2. For this purpose, the generator should be run in a solvent, which dissolves the substance residue and does not harm the components. The rotor and stator are cleaned as the solvent is pumped through the generator.
  3. Please ensure that all cleaning processes are compatible with 316SS and PTFE.
  4. For a more thorough cleaning it is recommended that your generator be disassembled and cleaned via one of the following processed;
    • Chemical process - Germicidal solutions (formalin, phenol, alcohol etc.) can disinfect in most cases.
      • Residues of the germicide must subsequently be removed with sterilized water.
      • Please ensure that all chemical processes are compatible with 316SS and PTFE.       
      • Sterilizing by humid heat – This means sterilizing with steam at a pressure of 2 bar above atmosphere and a temperature of 120oC.
        • PRO Generators are heat resistant up to 390° F / 198° C.
      • Sterilizing by hot air - Hot air sterilization is normally carried out at 160 to 190oC.
        • PRO Generators are heat resistant up to 390° F / 198° C
      • Flaming - This method can be used, however, it is only effective on external surfaces.
        • PRO Generators are heat resistant up to 390° F / 198° C



    PRO Generator Probes PRO Quick Connect Generator Maintenance
    Regular maintenance and inspection / replacement of wearing generator parts is recommended and will thereby extend the life of this valuable piece of equipment.
    **CAUTION**RUNNING THE GENERATOR WITH MISSING OR WORN COMPONENTS CAN CAUSE DAMAGE TO THE GENERATOR AND/OR HOMOGENIZER MOTOR UNIT.


    Perform an upper washer check:
    1. Is the upper white Polytetrafluoroethylene (PTFE) washer missing? These tend to get misplaced when cleaning and often the generator is reassembled without the PTFE washer.
    2. Is the white PTFE washer worn?
    3. If you answered yes to either of these questions, then you will need to order replacement upper PTFE washers. 
    4. Find the correct lower bearing part number for your generator.        
      • Refer to the Generator Spare Parts Section of your manual
      • Visit our generator replacement parts page on our website.
      • Speak with a sales consultant at PRO Scientific, P#203-267-4600, sales@proscientific.com, and they will be happy to assist you in finding the correct replacement part for your generator.

    Perform a lower bearing maintenance check:
    1. It is time to replace your lower Polytetrafluoroethylene (PTFE) bearing if...
      • The inside diameter of the lower PTFE bearing fits loosely on the outside diameter of the shaft
      • And/or you are able to wiggle and tilt the PTFE bearing
    2. Find the correct lower bearing part number for your generator.
      • Refer to the Generator Spare Parts Section of your manual
      • Visit our generator replacement parts page on our website.
      • Speak with a sales consultant at PRO Scientific, P#203-267-4600, sales@proscientific.com, and they will be happy to assist you in finding the correct replacement part for your generator.


    Upper SS bearing maintenance check:
    Replacement of upper stainless steel bearing should be performed by PRO Scientific Service technician or authorized distributor.
    Contact PRO Scientific service department, at P#203-267-4600, sales@proscientific.com , and request a return authorization for generator.


    Mid-Bearing maintenance check:
    1. All generators that are 120mm in length and longer contain a mid-bearing, which is located inside the generator approximately half way up the shaft.
    2. The mid-bearing must be lubricated by the sample/liquid during processing, therefore the sample volume should cover about half the length of the generator.
    3. Replacement of mid-bearing should be performed by PRO Scientific Service technician or authorized distributor.
    4. Contact PRO Scientific service department, at P#203-267-4600, sales@proscientific.com, and request a return authorization for generator



    Multi-Gen 7 Generator Probe MULTI-GEN Generator Maintenance:Multi-Gen 7XL Generator Probes

      Regular maintenance and inspection / replacement of lower bearings is recommended and will thereby extend the life of this valuable piece of equipment.



      Perform a lower bearing maintenance check:
      1. It is time to replace your lower Polytetrafluoroethylene (PTFE) bearing if...
        • The inside diameter of the lower PTFE bearing fits loosely on the outside diameter of the shaft
        • And/or you are able to wiggle and tilt the PTFE bearing
        • This item is the same for all PRO MULTI-GEN Generators. You will need to order part number PRO-03-11703P (6 pack of MULTI-GEN lower PTFE bearings).
        • Visit our generator replacement parts page on our website.





      For additional PRO Homogenizer Motor Unit Maintenance Click Here




      Request a Quote

                 



        Tuesday, November 11, 2014

        PRO Homogenizer Package Kits


        PRO Homogenizer Packages


        PRO Scientific offers a variety of economical homogenizer package kit which have been preselected to better assist you in choosing a homogenizer setup.

        Use the following .pdf flow chart to select a homogenizer package kit to best suit your needs.



        See all of our PRO Homogenizers


        Request a Quote


                

        Tuesday, October 28, 2014

        Ideal Tissue Homogenizer

        Tissue Homogenizer

        Tissue Homogenizer

        PRO Homogenizer are ideal tissue homogenizers 

        • Provide consistent results with our PRO Quick Connect Generator Probes
        • Keep your samples at ambient temperature with little to no heat emission
        • Easy and Convenient with no special tubes or beads to buy
        • Can eliminate cross-contamination between samples
        • Variable speed allows for maxiumum homogenization or if necessary for preservation of organelles, large molecules and viruses
        • Most homogenization can be complete in a matter of seconds

        Need some more information on tissue homogenization?
        Homogenizer Package Kits 
        Economical
        Selection based on tube size
        For processing in tubes from .5ml up to 50ml
        Quick & effective
        Get the results you want each and every time
        Built to last


        Request a Quote
        Multi-Sample Homogenization Systems
        Econcomical Homogenization: Mulit-Gen 7XL 
        Rapid Multi-Sample: Multi-Prep
        Two-Step Homogenization: DPS-20

        Request a Quote
        Here are just some of the many application references of tissue homogenizing with a PRO Scientific Homogenizer:

        Vanilloid receptor homogenization – PRO200 Homogenizer
        Fish tissue homogenization – PRO250 Homogenizer
        Brain tissue homogenization – PRO250 Homogenizer
        Frozen human pubococcygeus muscle tissue homogenization – PRO Homogenizer
        Prostrate tissue homogenization – PRO200 Homogenizer
        Mud snail homogenization – PRO200 Homogenizer
        Myocardial tissue homogenization – PRO200 Homogenizer
        Muscle - hind limb tissue – PRO200 Homogenizer

        See all of our PRO Homogenizers



                

        Friday, October 17, 2014

        Homogenizing maintenance and support.

        Need help with your homogenizing equipment?

        It is important to regularly clean and inspect the "wearing" parts on your PRO generator/probe to maintain it in top working condition.


        Need assistance taking apart and maintaining your generator probes?

        Lean more
        Have questions on your particularhomogenizing application or need service on your equipment?
        Contact us

                

        Thursday, August 21, 2014

        Two-Step & Rapid Multi-Sample Homogenizing Systems

        PRO Scientific offers two homogenizing options to free up your valuable lab time and significantly increase your laboratory productivity. 

        Multi-Prep: Rapid Multi-Sample Homogenizer

        Homogenize 6 samples at a time, up to 300 samples per hour.
        Automated walk-away homogenizing.

        Download Catalogs:
        Multi-Prep Flier      Multi-Prep How it Works
        • Rapid automated multi-sample homogenizing
        • Process up to 6 samples at the same time
        • Samples homogenized in seconds
        • Store up to 10 different programs
        • Easy Load Spider Tube Racks 15ml and 50ml tubes
        • Can perform 60 oscillations per minute
        • Automated engagement & disengagement of Probes
        • Multi-Prep Probes ensure great results while avoiding cross contamination
        • Compact design, occupies small footprint
        Learn More

        DPS-20 Two-Step Automated Homogenizing System

        Double your homogenizing efficiency by combining two methods of homogenizing, mechanical and ultrasonic into one automate system.
        Download Catalogs:
        Multi-Prep Flier      Multi-Prep How it Works
        • Three-in-one design allows the system to be used in any of the following automated configurations
          • Automated Ultrasonic Homogenizer
          • Automated Mechanical Homogenizer
          • Automate Mechanical & Ultrasonic Two-Step Homogenizer
        • Rapid automated multi-sample homogenizing
        • Process up to 20 in one run
        • Samples homogenized in seconds
        • Store up to 10 different programs
        • Cooling Carousel Tube Rack for 1.5ml, 15ml and 50ml tubes
        • Two option rinse cycles for cross contamination concerns
        Learn More